Sperm nuclear histone H2B: correlation with sperm DNA denaturation and DNA stainability
نویسندگان
چکیده
منابع مشابه
Identification of a Sperm Nuclear Annulus: A Sperm DNA Anchorl
We report the isolation and characterization of a new nuclear structure from spermatozoa of the golden Syrian hamster which we term the nuclear annulus. The nuclear annul us was located at the implantation fossa, the point at which the tail is joined to the sperm head, inside the nucleus adjacent to the inner nuclear envelope. Extraction of sperm nuclei with 2 M NaC1 and 10 mM dithiothreitol ca...
متن کاملRaman Analysis of Sperm Nuclear DNA Integrity
Raman Spectroscopy was evaluated as a non-invasive method of analysis of sperm DNA and the influence of UV irradiation on the sperm. The results show that Raman Spectroscopy, combined with multivariate analysis provide the reproducible and accurate information on DNA of sperm and the effect and location of damage.
متن کاملHuman sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics.
Reports indicate an increase in the incidence of DNA fragmentation in male factor infertility and its role in the outcome of assisted reproductive techniques (ART). However, reports are conflicting between the relationships of sperm DNA integrity with conventional semen parameters. We examined the relationship between conventional sperm parameters and DNA integrity using acridine orange (AO) te...
متن کاملP-37: Effect of Gibberellic Acid on Sperm DNA Double Strand Integrity, Nuclear Maturity and Motility
Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...
متن کاملP-56: Silymarin Protected The Sperm Nuclear Maturity and DNA Integrity in Varicocelized Rats
Background: Varicoceles are the abnormal tortuosity and dilatation of the veins of the pampiniform plexus that drain the testis. Recently, studies evaluating the role of oxidative stress in male infertility have shown that infertile men with varicocele have elevated levels of sperm and testicular tissue-derived reactive oxygen species (ROS). Therefore current study was designed to evaluate prot...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Asian Journal of Andrology
سال: 2008
ISSN: 1008-682X,1745-7262
DOI: 10.1111/j.1745-7262.2008.00415.x